The total ribosomal footprints were obtained from a mix of flower buds and open flowers of Col-0 and dweorg1 plants grown in the greenhouse, as described in Planchard et al.25 (link). The total RNA and ribosome footprints were depleted of rRNAs using the RiboMinus™ Plant Kit for RNA-Seq (invitrogen), following the manufacturer’s instructions. The sequencing libraries of total RNA and ribosomal footprints of two biological repeats were prepared using the NEXTflex Rapid Directional RNA-Seq Kit (Bioo Scientific) and the NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific), respectively. Next-generation sequencing was performed by the sequencing facility of the Institut de Biologie Intégrative de la Cellule (Gif-sur-Yvette, France) using Illumina NextSeq technology (single end, 75 nt). Bioinformatic analysis was performed as described in Planchard et al.25 (link).
All experimental research did fully comply with the relevant institutional, national, and international guidelines and legislation.
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