Cell Cycle Analysis of Trabectedin and Propranolol

HeLa, OV2008, Caov-3, and SK-OV-3 cell lines were seeded in 60 mm dishes at a density of 300,000 cells/well and exposed to trabectedin (IC₅₀) and/or propranolol (HeLa - Caov-3: 50 μM, OV2008 - SK-OV-3: 100 µM) alone or in combination in the absence or presence of 10 µM of NE. Afterward, the cells were harvested, washed twice in cold PBS, fixed in 70% ethanol, and stored at −20°C until analysis. The cell cycle modulation by drugs was evaluated by propidium iodide (PI) staining, as previously described (Danza et al., 2021 (link)). Then, flow cytometry (FCM) analysis was performed by using the Attune NxT Acoustic Focusing Cytometer (Thermo Fisher Scientific, MA, United States), and cell cycle phases were analyzed by the Attune NxT Analysis Software (Thermo Fisher Scientific, MA, United States).