Combination Therapy for Cancer Treatment

ONC201 was administered orally in 10:70:20 DMSO:PBS:Cremphor El as described [4 (link)] and treated weekly at the indicated doses. Bevacizumab was procured from the Fox Chase Cancer Center pharmacy and diluted in PBS. Bevacizumab was administered through retro-orbital injections every other week at a dose of 5 mg/kg. Regorafenib was procured from MedChemExpress (HY-1031) and administered orally at 10 mg/kg per day dissolved in PBS for at least 22 days. Anti-murine VEGF-A inhibitory antibody (Biolegend 512,808) was administered at 10 micrograms by i.p. twice weekly. Mouse body weight was observed every 3 days and when body weight began to drop Regorafenib dosing was stopped.