Liposomal Benzoporphyrin Derivative for Photodynamic Therapy

PEG-coated liposomal benzoporphyrin derivative (Lipo-BPD) was used for PDP in this study. Briefly, lipo-BPD was synthesized as per the conventional thin film hydration method. All lipids were purchased from Avanti Polar Lipids. Firstly, DPPC, cholesterol, DSPE-mPEG₂₀₀₀ and Benzoporphyrin Derivative Monoacid Ring A (US Pharmacopeia) were mixed together in chloroform at a molar ratio of 0.66:0.289:0.03:0.01 (27 (link)). 1X DPBS was used to hydrate the thin film at 42°C and extrusion was performed through 100 nm polycarbonate membranes (Whatman).

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Bhandari C., Fakhry J., Eroy M., Song J.J., Samkoe K., Hasan T., Hoyt K, & Obaid G. (2022). Towards Photodynamic Image-Guided Surgery of Head and Neck Tumors: Photodynamic Priming Improves Delivery and Diagnostic Accuracy of Cetuximab-IRDye800CW. Frontiers in Oncology, 12, 853660.

Publication 2022

100 nm polycarbonate membranes

Chloroform Cholesterol Dspe Lipids Lipo Liposomal Membranes Molar Polycarbonate

Corresponding Organization : The University of Texas at Dallas

Other organizations : Dartmouth College, Harvard University, Massachusetts Institute of Technology, Massachusetts General Hospital, Harvard–MIT Division of Health Sciences and Technology

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Variable analysis

independent variables

Lipid composition (DPPC, cholesterol, DSPE-mPEG2000, Benzoporphyrin Derivative Monoacid Ring A)

dependent variables

Liposomal Benzoporphyrin Derivative (Lipo-BPD) synthesis

control variables

Thin film hydration method
Extrusion through 100 nm polycarbonate membranes
1X DPBS used to hydrate the thin film at 42°C

controls

No positive or negative controls were explicitly mentioned in the provided information.

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