Conditional Lineage Tracing in Mice

Cdh5-CreER^T2 (Wang et al, 2010 (link)) and Ai14 (R26-tdTom) (Madisen et al, 2010 (link)) mice described previously were analyzed on a C57BL/6J background. The morning of vaginal plug detection was considered as embryonic day 0 (E0). For inducing recombination during embryonic development, a single dose of 1 mg of 4-hydroxytamoxifen (4-OHT, H7904; Sigma-Aldrich, ≥98% Z [trans] isomer) or tamoxifen (tam, T5648; Sigma-Aldrich) dissolved in peanut oil (10 mg/ml) was administered intraperitoneally to pregnant females at a defined developmental stage as indicated in the figures and figure legends. All experimental procedures were approved by the Uppsala Animal Experiment Ethics Board (permit numbers 130/15 and 5.8.18-06383/2020) and performed in compliance with all relevant Swedish regulations.

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Zhang Y., Ortsäter H., Martinez-Corral I, & Mäkinen T. (2022). Cdh5-lineage–independent origin of dermal lymphatics shown by temporally restricted lineage tracing. Life Science Alliance, 5(11), e202201561.

Publication 2022

4-hydroxytamoxifen (4-OHT, H7904;

4 oht Cdh5 Embryonic Embryonic development Hydroxytamoxifen Isomer Mice Peanut oil Pregnant females Recombination Vaginal

Corresponding Organization : Uppsala University

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Variable analysis

independent variables

Dose of 4-hydroxytamoxifen (4-OHT) or tamoxifen (tam) administered intraperitoneally to pregnant females

dependent variables

Recombination during embryonic development

control variables

Mouse strain (C57BL/6J background)
Mouse genotypes (Cdh5-CreER^T2 and Ai14 (R26-tdTom))
Timing of analysis (morning of vaginal plug detection as embryonic day 0 (E0))

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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