Blocking IFNAR receptor in astrocytes and neurons

Astrocytes and cortical neurons were treated with 5000 U/ml IFNαB/D [37 (link)] 16 h before infection, and virus titers were determined by focus-forming assays. The IFNAR receptor was blocked using monoclonal antibodies as previously described [4 (link)]. In brief, cells were incubated for 2 h with either 10 μg/mL MAR1-5A3 (eBioscience, 16-5945-85 [38 (link)]) or IgGI κ isotype control (eBioscience 14-4714-85). After 2 h of incubation, the medium was removed and cells were infected for 1 h. After the infection, the inoculum was replaced with medium containing 10 μg/mL antibody.