The strain used in this study was L. theobromae LA-SOL3, isolated from Vitis vinifera in Peru. LA-SOL3 was one of the most aggressive strains in artificial inoculations trials of cv. Red Globe plants2 (link). The culture was routinely grown on Potato Dextrose Agar (PDA) medium (Merck, Germany) at 25 °C.
For DNA extraction, the mycelium harvested from a culture grown on Potato Dextrose Broth (PDB, Merck, Germany) at 25 °C for three days was ground in liquid nitrogen and DNA was extracted according to Möller et al.30 (link). For protein and RNA extraction, LA-SOL3 was inoculated into a 250 mL flask containing 50 mL of PDB and incubated at 25 °C or at 37 °C for 4 days, as described earlier31 (link). All assays were performed in triplicate.
For DNA extraction, the mycelium harvested from a culture grown on Potato Dextrose Broth (PDB, Merck, Germany) at 25 °C for three days was ground in liquid nitrogen and DNA was extracted according to Möller et al.30 (link). For protein and RNA extraction, LA-SOL3 was inoculated into a 250 mL flask containing 50 mL of PDB and incubated at 25 °C or at 37 °C for 4 days, as described earlier31 (link). All assays were performed in triplicate.
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