Ferroptosis Characterization Protocol

Five canonical characteristics of ferroptosis were detected, including cellular concentrations of iron and malondialdehyde (MDA), GPx and GST activities, and mitochondrial morphology. For the iron, MDA and GSH-oxidized glutathione (GSSG) content assay, an Iron Colorimetric Assay Kit (Elabscience, E-BC-K139-S), a Lipid Peroxidation MDA Assay Kit (Beyotime, S0131S), and a GSH and GSSG Assay Kit (Beyotime, S0053) were used according to the manufacturer protocols. GPx and GST activity assays were performed using a glutathione peroxidase (GSH-PX) assay kit (Nanjing Jiancheng Bioengineering Institute, A005-1-2) and GST activity assay kit (BC0355, Solarbio, Beijing, China) following manufacturer protocols. Morphological observations of mitochondria were conducted using a transmission electron microscope (TEM) FEI Tecnai F20 (FEI, Netherlands) as previously described (57 (link)).

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Zhang B., Wang Z., Zhang S., Zhong S., Sun Y, & Liu X. (2024). N6-methyloxyadenine-mediated detoxification and ferroptosis confer a trade-off between multi-fungicide resistance and fitness. mBio, 15(3), e03177-23.

Publication 2024

Iron Colorimetric Assay Kit Lipid Peroxidation MDA Assay Kit GSH and GSSG Assay Kit GSH-PX) assay kit GST activity assay kit FEI Tecnai F20

Corresponding Organization :

Other organizations : China Agricultural University, Sanya University, Northwest A&F University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Cellular concentrations of iron
Cellular concentrations of malondialdehyde (MDA)
GPx activity
GST activity
Mitochondrial morphology

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

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