Chromatin Immunoprecipitation Antibody Protocols

The antibodies used for chromatin immunoprecipitation, immunoblotting, and immunofluorescence staining were rabbit anti-phospho ATM/ATR substrate motif (Cell Signaling Technology, Danvers, USA), mouse anti-phospho ATM (Rockland, Pottstown, USA), mouse anti-HA, mouse anti-γH2AX (Merck Millipore), rabbit anti-RAD51 (Bio Academia,Japan), rabbit anti -RPA2 S4/8 and rabbit anti-INO80 (Bethyl, Montgomery, USA), mouse anti-RPA34 (Lab Vision, Fremont, USA), rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH) (Santa Cruz Biotechnology, USA), rabbit anti-H2AX (Abcam, UK), and mouse anti-β-actin (Sigma-Aldrich) and rabbit anti-ARP8 (Osakabe et al., 2014 (link)).

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Sun J., Shi L., Kinomura A., Fukuto A., Horikoshi Y., Oma Y., Harata M., Ikura M., Ikura T., Kanaar R, & Tashiro S. (2018). Distinct roles of ATM and ATR in the regulation of ARP8 phosphorylation to prevent chromosome translocations. eLife, 7, e32222.

Publication 2018

mouse anti-phospho ATM rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH) rabbit anti-H2AX mouse anti-β-actin

Actin Antibodies Chromatin immunoprecipitation Glyceraldehyde 3 phosphate dehydrogenase Immunofluorescence Ino80 Mouse Rabbit Rpa2 Vision

Corresponding Organization :

Other organizations : Hiroshima University, Tohoku University, Kyoto University

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Variable analysis

independent variables

The antibodies used for chromatin immunoprecipitation, immunoblotting, and immunofluorescence staining

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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