Annexin V-FITC/PI Apoptosis Assay

Apoptosis was determined using the Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis Kit (Beckman Coulter, Brea, CA, USA), as described.^{21 (link),22 (link)} Mean percentage of AnnexinV+/PI- (early apoptotic) and Annexin V+/PI+ (late apoptotic and/or dead) ± standard error of the mean (SEM) from one representative experiment is shown.

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Li X., Su Y., Hege K., Madlambayan G., Edwards H., Knight T., Polin L., Kushner J., Dzinic S.H., White K., Yang J., Miller R., Wang G., Zhao L., Wang Y., Lin H., Taub J.W, & Ge Y. (2020). The HDAC and PI3K dual inhibitor CUDC-907 synergistically enhances the antileukemic activity of venetoclax in preclinical models of acute myeloid leukemia. Haematologica, 106(5), 1262-1277.

Publication 2020

Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis Kit

Annexin v Apoptotic Fluorescein Isothiocyanate Propidium iodide

Corresponding Organization :

Other organizations : Jilin University, Wayne State University, Oakland University, The Barbara Ann Karmanos Cancer Institute, Children's Hospital of Michigan, First Hospital of Jilin University

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Variable analysis

independent variables

Apoptosis

dependent variables

Mean percentage of AnnexinV+/PI- (early apoptotic)
Mean percentage of Annexin V+/PI+ (late apoptotic and/or dead)

control variables

Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis Kit (Beckman Coulter, Brea, CA, USA)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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