Culturing THP-1, HeLa, and Ehrlichia chaffeensis

Human monocytic leukemia cells (THP-1; ATCC TIB-202) were propagated in RPMI 1640 with L-glutamine and 25 mM HEPES buffer (Invitrogen, Carlsbad, CA), supplemented with 10% fetal bovine serum, and incubated at 37°C in a 5% CO₂ atmosphere. Henrietta Lack’s cervical epithelial adenocarcinoma (HeLa) cells were propagated in MEM medium with Earle’s Salts and L-glutamine (Thermo Fisher Scientific, Waltham, MA), supplemented with 10% fetal bovine serum. E. chaffeensis (Arkansas strain) was cultivated in THP-1 cells as previously described (Byerly et al., 2022 (link)).

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Byerly C.D., Zhu B., Warwick P.A., Patterson L.L., Pittner N.A, & McBride J.W. (2024). Ehrlichia chaffeensis TRP120 ubiquitinates tumor suppressor APC to modulate Hippo and Wnt signaling. Frontiers in Cell and Developmental Biology, 12, 1327418.

Publication 2024

L-glutamine HEPES buffer Earle’s Salts and L-glutamine

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Variable analysis

independent variables

Cell lines used: Human monocytic leukemia cells (THP-1), Henrietta Lack's cervical epithelial adenocarcinoma (HeLa) cells

dependent variables

Propagation and incubation conditions of the cell lines

control variables

RPMI 1640 with L-glutamine and 25 mM HEPES buffer (Invitrogen, Carlsbad, CA), supplemented with 10% fetal bovine serum
MEM medium with Earle's Salts and L-glutamine (Thermo Fisher Scientific, Waltham, MA), supplemented with 10% fetal bovine serum
Incubation at 37°C in a 5% CO2 atmosphere

controls

Positive control: Cultivation of Ehrlichia chaffeensis (Arkansas strain) in THP-1 cells, as previously described (Byerly et al., 2022)

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