The EC2-EC3 domains of DSG2 (Swiss-Prot: Q14126.2) corresponding to Val149 to Ile386 was cloned using the restriction sites AgeI/KpnI in pHL-sec plasmid (Addgene plasmid # 99845) as described in [27 (link)]. It contains a Kozak sequence, a secretion signal sequence, and a C-terminal hexahistidine-tag (6×His-tag). This plasmid was used for mammalian cell transient transfection.
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