Adipose Tissue Fatty Acid Analysis

The fatty acid composition test was performed according to the previously reported method (21 (link)). Gas chromatography-mass spectrometry (GC-MS 7890B-5977A, Agilent, United States) was used to detect the fatty acid composition. Then, 100 mg of adipose tissue was homogenized, 2 ml of n-hexane added, and it was shaken for 30 min at 50°C. Next, 3 ml of methanol solution (0.4 moL/L) was added and then shaken for 30 min at 50°C. Lastly, 1 ml of water was mixed with 2 ml of n-hexane and shaken for 20 min at (22 ± 3)°C. Afterward, the solution was allowed to stand for stratification, and the upper layer was separated for gas injection detection. Chromatographic column: DB-23 (30 m × 320 μm × 0.25 μm), the carrier gas was helium, inlet temperature was 250°C; the split ratio was 1/5; injection volume was 1 μl, detector temperature was 230°C, the column oven temperature was 50°C for 1 min, 25°C/min to 175°C, 4°C/min to 230°C for 24.75 min.