We used Tet-On 3G HEK293 cells (ClonTech), Tet-On HEK293T (from JD
Boeke40 (link)), Tet-On
3G Hela (ClonTech), HEK293FT (AJ Holland), hTERT-RPE1puroS (from
AJ Holland71 ), and
hTERT-RPE1puroS-Cas9 (from AJ Holland71 ). RPE cells have been authenticated
by STR profiling. Cells were grown in DMEM (293, HeLa) or DMEM/F12 with 1.5%
sodium bicarbonate (RPE) with 10% Tetracycline-free Fetal Bovine Serum
(Takara Bio USA). Cells were cultured at 37C, 5% CO2. Antibiotic
selection was performed with puromycin (1 μg/ml), G418 (400
μg/ml), or blasticidin (10 μg/ml). Doxycycline was used at 1
μg/ml unless otherwise stated. Cells were tested and mycoplasma
negative.
Boeke40 (link)), Tet-On
3G Hela (ClonTech), HEK293FT (AJ Holland), hTERT-RPE1puroS (from
AJ Holland71 ), and
hTERT-RPE1puroS-Cas9 (from AJ Holland71 ). RPE cells have been authenticated
by STR profiling. Cells were grown in DMEM (293, HeLa) or DMEM/F12 with 1.5%
sodium bicarbonate (RPE) with 10% Tetracycline-free Fetal Bovine Serum
(Takara Bio USA). Cells were cultured at 37C, 5% CO2. Antibiotic
selection was performed with puromycin (1 μg/ml), G418 (400
μg/ml), or blasticidin (10 μg/ml). Doxycycline was used at 1
μg/ml unless otherwise stated. Cells were tested and mycoplasma
negative.
