After a centrifugation step of 150 mL cultures of each strain, DNA extractions were performed on the pellets using the ZymoBIOMICS DNA mini kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s protocol. The Macherey Nagel genomic DNA and total RNA purification kit with NucleoBond AXG20 columns and buffer set III (Macherey-Nagel, PA, USA) was employed for PMC 881.14 and PMC 884.14. Metagenome sequencing was performed by GenoScreen (GenoScreen, Lille, France) using the Nextera XT DNA sample preparation kit (Illumina) for 2 × 250 bp and using a SMRT2 cell (PacBio) Illumina and Pacbio raw reads were corrected using SPAdes 3.12 and Canu 1.8, respectively, before the assembly performed with Unicycler hybrid-assembler, with default parameters [28 (link),29 (link),30 (link)]. For each new assembly, scaffolds were binned using MyCC (k-mer size = 4, minimal sequence size = 1000) and taxonomically annotated using CAT [31 (link)]. Congruent data between both methodologies allowed to characterize the cyanobacteria draft genomes.
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