Human breast cancer cell lines MCF10A, MDA-MB-468 (MDA-468), BT20, and MDA-MB-231 (MDA-231) were purchased from ATCC (Manassas, VA, USA) and cultured as instructed. Bone-metastatic MDA-MB-231BoM (231-BoM), and brain-metastatic MDA-MB-231BrM (231-BrM) cell lines are from the Massagué laboratory and were cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin [43 (link), 44 (link)]. Trastuzumab-resistant BT474 (BT474-TtzmR) were a kind gift from Dr. Dihua Yu [45 (link)]. All cell lines have been authenticated using the standard method, and tested for mycoplasma. If tested positive for mycoplasma contamination, cells were treated until free of contamination before use. Pacritinib, ruxolitinib, sonidegib, and vismodegib were obtained from AdooQ Bioscience (Irvine, CA, USA) and stock solutions were prepared using dimethyl sulfoxide (DMSO) for administration in cell culture.
Protocol detail
Breast Cancer Cell Lines and Inhibitors
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Bone
Brain
Breast cancer cell lines
Cell culture
Cell lines
Cells
Dimethyl sulfoxide
Fetal bovine serum
Human
Mycoplasma
Pacritinib
Penicillin
Ruxolitinib
Sonidegib
Streptomycin
Trastuzumab
Vismodegib
Corresponding Organization :
Other organizations : Wake Forest University, University of Maryland, Baltimore
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Variable analysis
independent variables
- Pacritinib
- Ruxolitinib
- Sonidegib
- Vismodegib
- Cell growth/proliferation
- Cell signaling
- Tumor metastasis
- Cell lines (MCF10A, MDA-MB-468, BT20, MDA-MB-231, MDA-MB-231BoM, MDA-MB-231BrM, BT474-TtzmR)
- Culture conditions (DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin)
- Mycoplasma testing and treatment
- Not specified
- DMSO (vehicle control)
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