Examining ACE2KO Mice on High-Fat Diet

Ace2^−/y mutant mice (ACE2KO) backcrossed into the C57BL/6 background for at least eight generations were used in the current study (18 (link),21 (link),22 (link)). All experiments were performed in accordance with University of Alberta institutional guidelines, which conform to guidelines published by the Canadian Council on Animal Care and the Guide for the Care and Use of Laboratory Animals published by the U.S. National Institutes of Health (revised 2011). Male wild-type (WT) and ACE2KO mice were fed either a high-fat diet (HFD) (45% kilocalories from fat) or a control diet (CON) (10% kilocalories from fat) from weaning to 6 months of age. ALZET microosmotic pumps (Model 1002; DURECT Corporation) were implanted subcutaneously in ACE2KO-HFD mice to deliver Ang 1-7 (24 μg/kg/h) or saline (control) for 4 weeks (21 (link)). All mice were studied at 6 months of age. Epicardial adipose tissues (EATs) were collected under a stereo microscope after removal of the pericardium and pericardial fat.

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Patel V.B., Mori J., McLean B.A., Basu R., Das S.K., Ramprasath T., Parajuli N., Penninger J.M., Grant M.B., Lopaschuk G.D, & Oudit G.Y. (2015). ACE2 Deficiency Worsens Epicardial Adipose Tissue Inflammation and Cardiac Dysfunction in Response to Diet-Induced Obesity. Diabetes, 65(1), 85-95.

Publication 2015

ALZET microosmotic pumps (Model 1002;

Ace2 Ang 1 7 Animal Diet High fat diet Laboratory animals Male Mice Microscope Pericardial Saline

Corresponding Organization : University of Alberta

Other organizations : Austrian Academy of Sciences, Institute of Molecular Biotechnology, Indiana University – Purdue University Indianapolis

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Variable analysis

independent variables

Diet (high-fat diet (HFD) or control diet (CON))
Ang 1-7 treatment (Ang 1-7 or saline control)

dependent variables

Epicardial adipose tissue (EAT) collection

control variables

Mouse strain (Ace2−/y mutant mice (ACE2KO) backcrossed into the C57BL/6 background for at least eight generations)
Mouse sex (male)
Mouse age (6 months)

controls

Positive control: Wild-type (WT) mice fed a control diet (CON)
Negative control: ACE2KO mice fed a control diet (CON)

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