Histone ChIP was performed as previously described with modifications (11 (link)). Protein A magnetic beads (10002D; Invitrogen), anti-H3 (ab176842; Abcam), and anti-H3K4me1 (ab176877; Abcam) were used. After immunoprecipitation, the eluted DNA was quantified by qPCR with primers listed in SI Appendix, Table S1. Data were normalized to 1% of input and presented as the ratio of H3K4me1 to H3.
Free full text: Click here