The assay was performed using susceptibility test disks (Oxoid), according to 2010 CLSI guidelines. Bacteria cultured in ampicillin (0.625 μg/ml) at 37°C for 16 hours were adjusted to a 0.5 McFarland standard. These bacteria were uniformly inoculated with Mueller-Hinton agar, and then, a 30-μg cefoxitin disk was placed at the center of the disk. After overnight incubation at 37°C, zone of inhibition was detected. Zone of inhibition 18 was defined as a positive threshold. Klebsiella pneumoniae ATCC700603 and Enterobacter cloacae ATCC13047 were used as negative and positive controls, respectively.
Protocol detail
Cefoxitin Disk Diffusion Assay
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Agar
Ampicillin
Assay
Bacteria
Cefoxitin
Enterobacter cloacae
Inhibition
Klebsiella pneumoniae
Susceptibility
Corresponding Organization : Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
Other organizations : Jinan University
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Variable analysis
independent variables
- Ampicillin (0.625 μg/ml)
- Zone of inhibition
- Susceptibility test disks (Oxoid)
- 2010 CLSI guidelines
- Mueller-Hinton agar
- 30-μg cefoxitin disk
- Incubation at 37°C for 16 hours
- 0.5 McFarland standard
- Klebsiella pneumoniae ATCC700603
- Enterobacter cloacae ATCC13047
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