The SKOV3 cell line (ATCC, USA) was used as an in vitro model for OvCa. Cells were grown in Roswell Park Memorial Institute (RPMI) media supplemented with 10% FBS (Gibco) and 1% penicillin/streptomycin (Gibco). Cell lines were cultured at 37 °C, in 5% CO2 environment. siRNA targeted to RAD51AP1 (SMARTpool: ON-TARGETplus, Dharmacon, CO, USA) was used to suppress RAD51AP1 gene/protein expression in SKOV3 cells, as previously described [3 (link)].
Filipe A., Katopodis P., Chudasama D., Kerslake R., Jeyaneethi J., Anikin V., Silva E., Kyrou I., Randeva H.S., Sisu C., Hall M, & Karteris E. (2022). Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro. Journal of Personalized Medicine, 12(2), 201.
Other organizations :
Brunel University of London, Harefield Hospital, Royal Brompton & Harefield NHS Foundation Trust, Sechenov University, University Hospitals Coventry and Warwickshire NHS Trust, University of Warwick, Coventry University, Aston University, Agricultural University of Athens, Mount Vernon Cancer Centre
SiRNA targeted to RAD51AP1 (SMARTpool: ON-TARGETplus, Dharmacon, CO, USA)
dependent variables
RAD51AP1 gene/protein expression in SKOV3 cells
control variables
SKOV3 cell line (ATCC, USA)
Roswell Park Memorial Institute (RPMI) media supplemented with 10% FBS (Gibco) and 1% penicillin/streptomycin (Gibco)
Cell culture at 37 °C, in 5% CO2 environment
controls
No positive or negative controls were explicitly mentioned in the provided information.
Annotations
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