Protein Extraction and Western Blot Analysis

About 50 mg of frozen tissue was homogenized for protein isolation. Aliquots of the homogenates were stored at −20 °C until further use for Western blot analysis. Protein isolation and Western blot analysis were implemented as described previously [25 (link)]. Western blot was performed utilizing commercially available antibodies for CXCL1, CXCL8 (R & D systems, Wiesbaden, Germany), and Stat 3 (Cell Science, Cologne, Germany) utilizing ECL reagent. The quantification of p-STAT-3 was performed by densitometric analysis using ImageJ software.

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Malik I.A, & Ramadori G. (2022). Interleukin-6-Production Is Responsible for Induction of Hepatic Synthesis of Several Chemokines as Acute-Phase Mediators in Two Animal Models: Possible Significance for Interpretation of Laboratory Changes in Severely Ill Patients. Biology, 11(3), 470.

Publication 2022

CXCL1 CXCL8

Antibodies Cell Cxcl1 Cxcl8 Densitometric Frozen Isolation Protein Tissue Western blot

Corresponding Organization : University of Göttingen

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Variable analysis

independent variables

Tissue homogenization

dependent variables

Protein expression levels of CXCL1, CXCL8, and p-STAT-3

control variables

Amount of frozen tissue used (about 50 mg)
Storage conditions of homogenates (-20 °C)
Commercially available antibodies used for Western blot analysis

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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