Eutrema salsugineum Transcriptome Profiling

Seeds of Eutrema salsugineum (Pall.) Al-Shehbaz and Warwick, accession Shandong, were originally collected by Ziyi Cao in the Yellow River Delta area near Dongying City, Shandong province, China, at ∼37°19′N and 118°28′E, and were subsequently inbred for multiple generations. High molecular weight nuclei were isolated from aerial tissue of 4-week-old soil-grown plants using an established protocol (Luo and Wing, 2003 (link)), and DNA was extracted using the Qiagen Genomic-tip System, modified for use with plant tissue¹. Five RNA libraries (CFGU, CFGW, CFHG, CFHH, CFHI) were generated from the following tissues for sequencing (all grown at 21°C under a light regime of 16 h light and 8 h dark): (1) 17-day-old whole seedlings grown on 0.1× Murashige and Skoog (MS) media (Murashige and Skoog, 1962 (link)) and 1% sucrose; (2) combined roots and shoots of 5-week-old plants grown hydroponically in a nutrient solution (Gibeaut et al., 1997 (link)) (modified by removing Na₂O₃Si); (3) combined roots and shoots of 5-week-old plants grown hydroponically in a nutrient solution (Gibeaut et al., 1997 (link)) (modified by removing Na₂O₃Si), after 6 h of treatment with 225 mMNaCl; (4) aerial portions of 4-week-old plants grown in soil, after 6 h at 4°C; (5) flowers of 4-week-oldplants grown in soil. RNA was isolated using TRIzol².