Polysaccharide and Enzyme Assay Protocols

Amylose, 2-hydroxyethyl-cellulose (Sigma 434965, molar 2-hydroxyethyl substitution 2.5 mol per mol cellulose), curdlan, laminarin, amylopectin, pectolyase from A. japonicus and α-amylase from bovine pancreas were obtained from Sigma (Brøndby, Denmark). All other polysaccharides were obtained from Megazyme (Bray, Ireland). The enzymes used are listed with the supplier in Table 2. The dyes reactive red 4, reactive blue 4, reactive green 19 and reactive yellow 2, cross-linker 1,4-butanediol diglycidyl ether, NaOH and all salts for buffers were obtained from Sigma (Brøndby, Denmark). Two pathogenic fungi C. acutatum (isolate SA 0-1) and P. expansum (isolate IK2020) and the apple pomace media were kindly provided by Birgit Jensen and Daniel Buchvaldt Amby (Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg, Denmark).