This assay of outside/in leak is described in depth by Veazey et al.18 (link) Briefly here, C57/B6 mice were administered 10 μg poly(I:C) (InVivoGen, San Diego, CA, USA) on days 0–2 to induce inflammation. On day 3, 0.2 mg of 4 kDa FITC–dextran (Sigma, St. Louis, MO, USA) was administered by oropharyngeal aspiration and mice were killed 1 h after 4 kDa FITC–dextran administration. BALF was collected as described earlier. Blood was collected via cardiac puncture and serum analyzed for FITC–dextran levels with a plate reader (Beckman Coulter DTX 880 Multimode Plate Reader).
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Quantifying Intestinal Permeability in Mice
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Corresponding Organization : University of Rochester
Other organizations : Greenwich Hospital, University of North Carolina System, MSD (United States)
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Variable analysis
independent variables
- Poly(I:C) administration on days 0-2 to induce inflammation
- FITC–dextran levels in serum
- Mouse strain (C57/B6)
- FITC–dextran dose (0.2 mg of 4 kDa FITC–dextran)
- Time between FITC–dextran administration and sample collection (1 h)
- No positive or negative controls were explicitly mentioned in the provided information.
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