Diabetes Cell Model and CGRP Treatment

The cell model with diabetes was induced by HG as previously described by Qiu^{22 (link), 23 (link)}. Briefly, BSMCs were grown in DMEM supplemented with 5 mM D-glucose (normal glucose as a control), to mimic diabetes in vitro, the cells were maintained in DMEM with 25 mM D-glucose (Gibco/BRL) for 48 h as a HG treatment. BSMCs were divided into 8 groups: control group, HG group, HG + CGRP group, HG + CGRP + AA group, CGRP group, AA group, HG + CGRP + CGRP-8-37 group and HG + LY2228820 group. The relevant use concentrations were as follows: CGRP (20 pg/ml, Abcam, UK), AA (5 μM, Abcam, UK), CGRP-8-37 (2 μM, Abcam, UK) and LY2228820 (1 μM, Abcam, UK). The cells were incubated in the incubator with 5% CO₂ at 37 °C for 48 h.