Equine Muscle Metabolomics by UPLC-MS/MS

Approximately 100 mg of frozen muscle biopsy samples from exercised horses was sent on dry ice to Metabolon^®, Inc. for ultra-high performance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS) analysis using their DiscoveryHD4 comprehensive global metabolomics platform. Samples were prepared and analyzed as per the company’s protocol (Gall et al., 2010 (link)). For metabolite identification, retention time/index (RI), mass-to-charge ratio (m/z), and MS/MS spectra were compared against authenticated standards as part of a library that is curated and maintained by Metabolon^®, Inc. (Gall et al., 2010 (link)).

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Klein D.J., McKeever K.H., Mirek E.T, & Anthony T.G. (2020). Metabolomic Response of Equine Skeletal Muscle to Acute Fatiguing Exercise and Training. Frontiers in Physiology, 11, 110.

Publication 2020

DiscoveryHD4

Biopsy Dry ice Frozen High performance liquid chromatography Horses Library Ms ms Muscle Retention

Corresponding Organization : Rutgers, The State University of New Jersey

Other organizations : Rowan University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Metabolite profiles of muscle biopsy samples from exercised horses

control variables

Frozen muscle biopsy samples
Samples prepared and analyzed as per Metabolon's protocol
Metabolite identification using retention time/index, mass-to-charge ratio, and MS/MS spectra compared against authenticated standards in Metabolon's curated library

controls

Positive control: Authenticated standards in Metabolon's curated library
Negative control: Not explicitly mentioned

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