Protocol detail

Genotyping of FTCDNL1 SNPs

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We selected five of the most significant SNPs (rs7572473, rs12473679, rs17529497, rs7605378, and rs10203122) of FTCDNL1 based on a previous study with a minimum allele frequency of ≥ 1% in a Beijing Han Chinese population[17 (link)]. The FTCDNL1 gene structure is shown in Fig 1. Genotyping was performed by the TaqMan Allelic Discrimination assay (Applied Biosystems, Foster City, CA). The polymerase chain reaction (PCR) was accomplished by an ABI StepOnePlus Thermal Cycler. In a subsequent PCR, the fluorescence from specific probes was detected and analyzed through the System SDS software version 2.2.2 (Applied Biosystems).

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Lu H.F., Hung K.S., Hsu Y.W., Tai Y.T., Huang L.S., Wang Y.J., Wong H.S., Hsu Y.H, & Chang W.C. (2015). Association Study between the FTCDNL1 (FONG) and Susceptibility to Osteoporosis. PLoS ONE, 10(10), e0140549.

Publication 2015

TaqMan Allelic Discrimination assay StepOnePlus Thermal Cycler System SDS software version 2.2.2

Allelic Assay Chinese Discrimination Fluorescence probes Gene structure Polymerase chain reaction Snps

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Variable analysis

independent variables

Rs7572473
Rs12473679
Rs17529497
Rs7605378
Rs10203122

dependent variables

Fluorescence from specific probes

control variables

Minimum allele frequency of ≥ 1% in a Beijing Han Chinese population
TaqMan Allelic Discrimination assay (Applied Biosystems, Foster City, CA)
ABI StepOnePlus Thermal Cycler
System SDS software version 2.2.2 (Applied Biosystems)

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