SARS-CoV-2 Protein Detection by Immunoblotting

Immunoblotting was performed using the following antibodies: mouse anti-Flag monoclonal antibody (F1804, Sigma Aldrich, 1:2000); rabbit anti-SARS-CoV-2 N antibody (1:2000, 9103, Prosci); rabbit anti-SARS-CoV-2 S antibody (1:1000, PA5-81795, Invitrogen); rabbit anti-TDRD3 antibody (1:1000, Bethyl Laboratory); mouse anti-SMN antibody (1:2000, 610646, BD Biosciences); rabbit anti-G3BP1 antibody (1:1000, 1F1, Rhône-Poulenc Rorer, a kind gift from Dr Imed Gallouzi at McGill University) (87 (link)); rabbit anti-ASYM26 (1:1000, 13-0011, EpiCypher). Immunofluorescence was performed with the following antibodies: mouse anti-Flag monoclonal antibody (F1804, Sigma Aldrich, 1:500); rabbit anti-G3BP1 antibody (1F1, 1:500). Alexa Fluor–conjugated goat anti-rabbit, goat anti-mouse secondary antibodies were from Invitrogen. Protease inhibitor cocktail and protein phosphatase inhibitor cocktail were from Roche. MS023 (SML1555), sodium arsenite (S7400), Protein A-Sepharose (P3391), and PRMT5:MEP50 active complex (SRP0145) were purchased from Sigma Aldrich. Protein coimmunoprecipitation was performed using ANTI-FLAG M2 Affinity Gel (A2220, Sigma Aldrich).