SA-β-gal staining was performed as previously described (Wu et al., 2018 (link)). In brief, cells were fixed in fixation buffer (2% formaldehyde and 0.2% glutaraldehyde) for 5 min at RT and stained with fresh SA-β-gal staining solution [150 mmol/L NaCl, 2 mmol/L MgCl2, 40 mmol/L citric acid/Na phosphate buffer, 5 mmol/L K3(Fe[CN]6), 5 mmol/L K4(Fe[CN]6), 1 mg/mL X-gal (AMRESCO, 0428-25G)] at 37°C overnight in the dark. Images were captured using a digital camera combined with an optical microscope (Olympus) and the percentage of SA-β-gal-positive cells was determined with ImageJ.
He Y., Ji Q., Wu Z., Cai Y., Yin J., Zhang Y., Zhang S., Liu X., Zhang W., Liu G.H., Wang S., Song M, & Qu J. (2022). 4E-BP1 counteracts human mesenchymal stem cell senescence via maintaining mitochondrial homeostasis. Protein & Cell, 14(3), 202-216.
Buffer CameraCitric acidFormaldehyde Glutaraldehyde Mgcl2 Nacl Optical microscope Phosphate X gal
Corresponding Organization :
Other organizations :
University of Chinese Academy of Sciences, Institute of Zoology, Chinese Academy of Sciences, Institute of Biophysics, Center for Excellence in Brain Science and Intelligence Technology, Shanghai Center For Bioinformation Technology, Capital Medical University, Beijing Institute of Genomics, First People's Hospital of Chongqing
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