Culturing Mouse Neural Stem Cells

NSCs were harvested from embryonic C57BL/6J mouse brain (E14) as previously described [14 (link)]. Briefly, cells were resuspended in a DMEM/F12 medium containing B27 (2%, Gibco, USA), EGF (20 ng/ml, BioLegend, USA), bFGF (20 ng/ml, BioLegend, USA), and ITSS (10 μg/ml, Roche, Switzerland) and cultured in a cell incubator. The medium was changed every 3 d, and cells were passaged when the neurospheres grew to 50-100 μm diameter. Cells were plated on poly-D-lysine and laminin precoated coverslips for further experiments.

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Xu P., Shi X., Zhang X., Liu Q., Xie Y., Hong Y., Li J., Peng M., Liu X, & Xu G. (2019). Overexpression of BRCA1 in Neural Stem Cells Enhances Cell Survival and Functional Recovery after Transplantation into Experimental Ischemic Stroke. Oxidative Medicine and Cellular Longevity, 2019, 8739730.

Publication 2019

EGF bFGF

Brain C57bl mouse Cells Cultured cell Embryonic Laminin Lysine Poly

Corresponding Organization : Nanjing General Hospital of Nanjing Military Command

Other organizations : University of British Columbia, First Affiliated Hospital of Wannan Medical College, Nanjing University of Chinese Medicine

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Variable analysis

independent variables

Harvesting of NSCs from embryonic C57BL/6J mouse brain (E14)

dependent variables

Growth and proliferation of NSCs in culture
Plating of NSCs on poly-D-lysine and laminin precoated coverslips

control variables

DMEM/F12 medium containing B27 (2%, Gibco, USA), EGF (20 ng/ml, BioLegend, USA), bFGF (20 ng/ml, BioLegend, USA), and ITSS (10 μg/ml, Roche, Switzerland)
Cell incubator conditions
Passage of cells when neurospheres grew to 50-100 μm diameter

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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