Multi-Gene Mutation Detection in FFPE Samples

Specimens were preserved in formalin and were transferred for pathological diagnosis and genomic testing. The pathological diagnosis was evaluated after hematoxylin & eosin and immunohistochemistry staining. The DNA/RNA was extracted from formalin-fixed, paraffin-embedded specimens via an FFPE DNA Extraction Kit (Amoy Diagnostics). The genomic testing of all specimens was performed in an AmoyDx Multi-Gene Mutations Detection Kit (Amoy Diagnostics), which harbored DNA-based mutation and RNA-based fusion detection real-time PCR assays, and can detect the mutational status of EGFR, KRAS, BRAF, NRAS, HER2, PIK3CA, ALK, ROS1, MET, and RET simultaneously [22 (link)].