Experiments involving neonatal and adult mice were conducted according to the ethical guidelines for animal care of the Rostock University Medical Centre. Isolation of neonatal CMs was performed as described previously [30 (link)]. Following enzymatic digestions, cells were seeded on 8 well chamber slides and cultured in DMEM supplemented with 10% FBS (Pan Biotech, Aidenbach, Germany) and 1% Zellshield (Biochrom) on 0.1% gelatin (Sigma Aldrich) coated surfaces.
Adult murine cardiomyocytes were isolated as described elsewhere [31 (link)]. Briefly, adult CM were obtained by injection of collagenase 2/4 solution (Sigma Aldrich) into the left ventricle. Digested heart tissue was cut into smaller pieces and gently triturated with a 1 mL pipette. Isolated cells were seeded on collagen-coated cell surfaces and cultured for one day before being subjected to fluorescence labelling.
Adult murine cardiomyocytes were isolated as described elsewhere [31 (link)]. Briefly, adult CM were obtained by injection of collagenase 2/4 solution (Sigma Aldrich) into the left ventricle. Digested heart tissue was cut into smaller pieces and gently triturated with a 1 mL pipette. Isolated cells were seeded on collagen-coated cell surfaces and cultured for one day before being subjected to fluorescence labelling.
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