Micro-CT Analysis of Bone Microstructure

Front paws and tibias from mice were imaged using a Skyscan 1172 micro-CT scanner (Bruker) using X-ray beam settings of 60 kV/167 μA with a 0.5-mm aluminium filter. Projections were taken every 0.45° at 580-ms exposure. Image volumes were reconstructed using the Feldkamp algorithm (NRecon 1.6.1.5, Bruker) having applied beam hardening correction. Trabecular bone parameters (bone volume to tissue volume (BV/TV), trabecular thickness (Tb.Th) and trabecular number (Tb.N)) of the tibia were analysed using CTAn software. One millimetre of bone (150 sections) in the metaphyseal region beneath the growth plate was analysed, and regions of interest (ROI) were selected by drawing around the trabecular network for each cross-sectional slice. Front paws were reconstructed, and MeshLab 1.3.2 was used to generate meshes which could then be scored for bone erosions as described previously [18 (link)].

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Fenton C.G., Webster J.M., Martin C.S., Fareed S., Wehmeyer C., Mackie H., Jones R., Seabright A.P., Lewis J.W., Lai Y.C., Goodyear C.S., Jones S.W., Cooper M.S., Lavery G.G., Langen R., Raza K, & Hardy R.S. (2019). Therapeutic glucocorticoids prevent bone loss but drive muscle wasting when administered in chronic polyarthritis. Arthritis Research & Therapy, 21, 182.

Publication 2019

Skyscan 1172 NRecon 1.6.1.5

Aluminium Beam Bone Bone tissue Ct scanner Growth plate Mice Tibia Trabecular X ray

Corresponding Organization :

Other organizations : University of Birmingham, University of Glasgow, Anzac Research Institute, University of Sydney, Maastricht University, Sandwell & West Birmingham Hospitals NHS Trust

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Variable analysis

independent variables

X-ray beam settings (60 kV/167 μA with a 0.5-mm aluminium filter)
Projection angle (every 0.45°)
Exposure time (580-ms)

dependent variables

Trabecular bone parameters (bone volume to tissue volume (BV/TV), trabecular thickness (Tb.Th) and trabecular number (Tb.N))
Bone erosions in front paws

control variables

Mouse front paws and tibias
Skyscan 1172 micro-CT scanner (Bruker)
Feldkamp algorithm (NRecon 1.6.1.5, Bruker) for image reconstruction
Beam hardening correction applied
1 mm of bone (150 sections) in the metaphyseal region beneath the growth plate analyzed
Regions of interest (ROI) selected by drawing around the trabecular network for each cross-sectional slice
MeshLab 1.3.2 used to generate meshes for front paws

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