Dietary Acetate and Carnitine Supplementation

Flies were maintained as previously described (Yoo et al, 2016 (link)). The fly food is composed of the following ingredients: 0.8% agar, 10% glucose, 4.5% corn flour, 3.72% dry yeast, 0.4% propionic acid, 0.3% butyl p‐hydroxybenzoate. For acetate supplementation experiments, acetate (FUJIFILM Wako) was added to the fly food to a final concentration of 333 or 500 mM. Carnitine (Tokyo Chemical Industry) was also added to the fly food to a final concentration of 100 mM.

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Okada M., Takano T., Ikegawa Y., Ciesielski H., Nishida H, & Yoo S.K. (2023). Oncogenic stress‐induced Netrin is a humoral signaling molecule that reprograms systemic metabolism in Drosophila. The EMBO Journal, 42(12), e111383.

Publication 2023

Carnitine

Acetate Agar Butyl p hydroxybenzoate Carnitine Corn flour Food Glucose Propionic acid Yeast

Corresponding Organization :

Other organizations : RIKEN Center for Biosystems Dynamics Research, Kyoto University, Kobe University

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Variable analysis

independent variables

Acetate concentration in fly food (333 mM or 500 mM)
Carnitine concentration in fly food (100 mM)

dependent variables

Not explicitly mentioned

control variables

Fly food composition (0.8% agar, 10% glucose, 4.5% corn flour, 3.72% dry yeast, 0.4% propionic acid, 0.3% butyl p‐hydroxybenzoate)
Fly maintenance as previously described (Yoo et al., 2016)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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