To directly test the impact of charcoal on carbonyl delivery, we utilized an in-line charcoal filter placed downstream of either conventional or MYO cigarettes. This filter, which consisted of charcoal contained in a 1 mL Supelco plastic tube (Bellefonte, PA, United States) held in place by deactivated glass wool (Restek, Bellefonte, PA, United States), was placed in-line between the cigarette and the pump (Figure 1). The charcoal used for all studies was Donau Carbon Alcarbon CI 60/30 × 70 (Donau Carbon US LLC, Dunnellon, FL, United States; provided gratis from the company), a product actively marketed for usage in cigarette filters.22 To compare to previous literature,7 (link) Anasorb CSC charcoal (SKC Inc., Eighty Four, PA, United States) was purchased.