MALDI-TOF-MS Protein Analysis Protocol

MALDI-TOF-MS was conducted on a
Bruker Microflex LRF instrument
with a microScout ion source. A saturated solution of sinapinic acid
(10 mg/mL in 70% acetonitrile in water +0.1% TFA) was used as the
matrix. Samples were prepared by a serial dilution of the matrix.
The solutions were dispensed onto a sample plate and dried at room
temperature. Apomyoglobin (MW = 16,952.27 Da) and aldolase (MW = 39, 211
Da) were used to calibrate the instrument.

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Zhang Z., Lynch C.J., Huo Y., Chakraborty S., Cremer P.S, & Mozhdehi D. (2024). Modulating Phase Behavior in Fatty Acid-Modified Elastin-like Polypeptides (FAMEs): Insights into the Impact of Lipid Length on Thermodynamics and Kinetics of Phase Separation. Journal of the American Chemical Society, 146(8), 5383-5392.

Publication 2024

Corresponding Organization : Pennsylvania State University

Other organizations : Institute for Excellence and Ethics

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Variable analysis

independent variables

Concentration of sinapinic acid in the matrix solution
Sample dilution of the matrix

dependent variables

Mass spectra of the samples

control variables

Bruker Microflex LRF instrument with a microScout ion source
Sinapinic acid as the matrix solution (10 mg/mL in 70% acetonitrile in water + 0.1% TFA)
Sample preparation method (dispensing and drying on a sample plate)
Apomyoglobin (MW = 16,952.27 Da) and aldolase (MW = 39,211 Da) used for instrument calibration

positive controls

Apomyoglobin (MW = 16,952.27 Da)
Aldolase (MW = 39,211 Da)

negative controls

Not explicitly mentioned

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