Total plasma triacylglycerol levels (L-Type TG M, Wako Diagnostics) and total plasma cholesterol levels were quantified enzymatically (Infinity Cholesterol Reagent, Thermo/Fisher). The distribution of cholesterol across lipoprotein classes was performed by fast-protein liquid chromatography (FPLC) using tandem superose-6 HR columns coupled with an online enzymatic cholesterol quantification as previously described (Warrier et al., 2015 (link); Brown et al., 2010 (link); Brown et al., 2008a (link); Brown et al., 2008b (link); Izem and Morton, 2009 (link); Helsley et al., 2016 (link)).
Other organizations :
Cleveland Clinic, University of Cincinnati, Harvard University, Brigham and Women's Hospital, University of California, Los Angeles, Ionis Pharmaceuticals (United States), Cleveland State University, University of Virginia, Wake Forest University
The distribution of cholesterol across lipoprotein classes
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