Immunohistochemical Evaluation of Angiogenesis

Tissue sample
preparation for
immunohistochemical evaluation was carried out in the Histology and
Tissue Analysis Core at Dell Pediatric Research Institute (DPRI) at
The University of Texas at Austin. The formalin-fixed tumor tissues
were embedded in paraffin wax, sectioned, and stained with an antibody
against CD-31 (Abcam, Cambridge, MA, USA) as a marker for angiogenesis
(n = 3).^{41 (link)} Slides were
then scanned, and images were taken using the ScanScope XT (Aperio
Technologies, Vista, CA, USA).

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Naguib Y.W., Rodriguez B.L., Li X., Hursting S.D., Williams RO I.I.I, & Cui Z. (2014). Solid Lipid Nanoparticle Formulations of Docetaxel Prepared with High Melting Point Triglycerides: In Vitro and in Vivo Evaluation. Molecular Pharmaceutics, 11(4), 1239-1249.

Publication 2014

antibodyagainst CD-31 ScanScope XT

Angiogenesis Austin Formalin Paraffin wax Tissue Tumor

Corresponding Organization :

Other organizations : The University of Texas at Austin

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Variable analysis

independent variables

Tissue sample preparation for immunohistochemical evaluation

dependent variables

CD-31 (Abcam, Cambridge, MA, USA) as a marker for angiogenesis

control variables

Formalin-fixed tumor tissues embedded in paraffin wax
Tissue sections
Staining with antibody against CD-31

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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