A broiler from each pen was selected at the end of the experiment then five mL of
blood samples were collected from the wing vein. All samples were centrifuged at
3,000×g for 15 minutes and serum samples were stored at 20°C for
further analysis. The biochemical parameters including glucose, triglyceride
(TG), cholesterol, LDL, high density lipoprotein (HDL), urea, uric acid, total
protein [26 (link)], albumin, aspartate
aminotransferase (AST), alanine aminotransferase (ALT) [27 (link)] and alkaline phosphatase (ALP) were determined by a
Technicon RA-1000 auto-analyzer according to the manufacturer’s
recommendations (Pars Azmoon, Tehran, Iran). In addition, the serum globulin
concentration was calculated by the subtraction between total protein and
albumin [23 (link)]. Enzyme activities,
including serum superoxide dismutase (SOD) [28 (link)], glutathione peroxidase (GPx), and total antioxidant capacity
(TAC) in serum were analyzed according to the method using the RANSEL and RANSOD
kits (Randox Laboratories, Crumlin, Antrim, UK). The serum MDA concentration in
the homogenates, was displayed as nmol/g, was determined by the Jo and Ahn
[29 (link)] method. Furthermore, the
catalase (CAT) concentration of blood was measured according to the method of
Aebi [30 (link)].
blood samples were collected from the wing vein. All samples were centrifuged at
3,000×g for 15 minutes and serum samples were stored at 20°C for
further analysis. The biochemical parameters including glucose, triglyceride
(TG), cholesterol, LDL, high density lipoprotein (HDL), urea, uric acid, total
protein [26 (link)], albumin, aspartate
aminotransferase (AST), alanine aminotransferase (ALT) [27 (link)] and alkaline phosphatase (ALP) were determined by a
Technicon RA-1000 auto-analyzer according to the manufacturer’s
recommendations (Pars Azmoon, Tehran, Iran). In addition, the serum globulin
concentration was calculated by the subtraction between total protein and
albumin [23 (link)]. Enzyme activities,
including serum superoxide dismutase (SOD) [28 (link)], glutathione peroxidase (GPx), and total antioxidant capacity
(TAC) in serum were analyzed according to the method using the RANSEL and RANSOD
kits (Randox Laboratories, Crumlin, Antrim, UK). The serum MDA concentration in
the homogenates, was displayed as nmol/g, was determined by the Jo and Ahn
[29 (link)] method. Furthermore, the
catalase (CAT) concentration of blood was measured according to the method of
Aebi [30 (link)].
