Transfection of Epithelial Cells with miR-16

RNA was extracted from six independent primary cultures of epithelial cells from C4HD tumors,^{9 (link)} transfected for 48 h with 30 nM pre-miR-16 or pre-miR-Control (Ambion). A detailed protocol is provided under Supplementary Materials and methods. Data were deposited into Gene Expression Omnibus^{76 (link)} (GSE73900).

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Venturutti L., Russo R.C., Rivas M., Mercogliano M., Izzo F., Oakley R., Pereyra M., De Martino M., Proietti C., Yankilevich P., Roa J., Guzmán P., Cortese E., Allemand D., Huang T., Charreau E., Cidlowski J., Schillaci R, & Elizalde P. (2016). MiR-16 mediates trastuzumab and lapatinib response in ErbB-2-positive breast and gastric cancer via its novel targets CCNJ and FUBP1. Oncogene, 35(48), 6189-6202.

Publication 2016

pre-miR-Control

Gene expression Primary cells cultures Tumors

Corresponding Organization : Consejo Nacional de Investigaciones Científicas y Técnicas

Other organizations : Cornell University, National Institute of Environmental Health Sciences, Hospital Fernández, Biomedicine Research Institute of Buenos Aires - CONICET - Partner Institute of the Max Planck Society, Pontificia Universidad Católica de Chile, Universidad de La Frontera, Advanced Center for Chronic Diseases, Instituto Universitario Aeronáutico

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Variable analysis

independent variables

Pre-miR-16
Pre-miR-Control (Ambion)

dependent variables

Gene expression

control variables

C4HD tumors
Primary cultures of epithelial cells
Transfection time (48 h)

controls

Pre-miR-Control (Ambion)

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