Substrates were immersed in 2 mg of dopamine.HCl per 1 mL of 10 mM Tris buffer (pH 8.5) typically overnight (12-18 h) and rinsed with water for further use. The polydopamine-coated substrates were transferred into trypsin solution (50 mM sodium phosphate buffer, pH 7.8) for overnight reaction (12-18 h). Hydrolysis tests of polydopamine-coated silicon and NHS-modified glass (μsurfaces Inc. Minneapolis, MN) were performed in 50 mM sodium phosphate buffer (pH 7.5) with 100 mM NaCl.
Protocol detail
Polydopamine Surface Modification and Hydrolysis
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Corresponding Organization : Northwestern University
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Variable analysis
independent variables
- Concentration of dopamine.HCl (2 mg/mL)
- Duration of polydopamine coating (12-18 h)
- Duration of trypsin treatment (12-18 h)
- Buffer composition (10 mM Tris buffer, pH 8.5; 50 mM sodium phosphate buffer, pH 7.8; 50 mM sodium phosphate buffer, pH 7.5)
- Hydrolysis of polydopamine-coated substrates (silicon and NHS-modified glass)
- Substrate materials (silicon and NHS-modified glass)
- Ionic strength (100 mM NaCl)
- Positive control: Not specified
- Negative control: Not specified
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