The SLAM microscope has previously been demonstrated and explained in several publications [13 (link)], [31 (link)], [32 (link)], [33 (link)], [34 (link)], [35 (link)], [36 (link)]. Briefly, this microscope employs a fiber laser with a center wavelength of 1030 nm, 20 MHz pulse repetition rate, and 378 fs pulses (Satsuma, Amplitude lasers). The laser is coupled into a photonic crystal fiber (NKT LMA-PM-15) to produce a supercontinuum of light which is then sent to a spatial light modulator based pulse compressor (MIIPSbox640, Biophotonics solutions) to compress the pulse duration down to 50 fs. The microscope uses two conjugated galvanometer mirrors for scanning, an Olympus objective lens (XLPLN25XWMP2), and four photon-counting photomultiplier tubes with the following filters (center wavelength / bandwidth): 370/10 nm, 450/60 nm, 555/30 nm, 610/60 nm, used for collection of THG, NADH, SHG, FAD signals, respectively. The average power at the sample was 14 mW and the pixel dwell time was 22 μs. Individual images have a pixel size of 500 nm over a total of 900 × 900 pixels, requiring ~18 s acquisition time.