Purified nuocytes were cultured for 2 - 5 days in RPMI supplemented with 10 ng/ml IL-33 and 10 ng/ml IL-7. Cells were washed and injected intravenously in PBS via the tail vein at 0.5 × 106 cells/mouse. Adoptive transfer was performed four hours after infection with N. brasiliensis, or 12 hours before the first cytokine administration of IL-25 i.p.
Protocol detail
Nuocyte Adoptive Transfer for Parasitic Infection
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Corresponding Organization :
Other organizations : MRC Laboratory of Molecular Biology, Trinity College Dublin
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Variable analysis
independent variables
- Culture duration (2 - 5 days)
- Cytokine treatment (10 ng/ml IL-33 and 10 ng/ml IL-7)
- Timing of adoptive transfer (4 hours after infection with N. brasiliensis, or 12 hours before the first cytokine administration of IL-25)
- Cell response (not explicitly mentioned)
- Culture medium (RPMI)
- Cell number (0.5 × 10^6 cells/mouse)
- Administration route (intravenous injection via the tail vein)
- Administration vehicle (PBS)
- Positive control: Not specified
- Negative control: Not specified
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