Pulmonary arterial blood flow imaging was obtained by pulmonary artery angiography (AlluraXper FD20 Philips). 5–7 ml of contrast was injected for the pulmonary angiography with hand injection with a syringe. The pulmonary circulation time was measured as previously described, by calculating the time taken for the contrast medium to reach from the initiation site of the pulmonary artery trunk to the left atrium through the pulmonary artery trunk and branches, pulmonary capillary, and pulmonary vein. The pulmonary circulation time was calculated for all of the survived rabbits at 120 min after the moment of successfully modeling, which was designed as the study end [6 (link)].
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