IL-2/α-IL-2 complexes were previously described to induce proliferation of T cells and NK cells in vivo in mice after intraperitoneally (i.p.) injection30 (link),31 (link). Complexes were prepared by mixing 2.5 μg recombinant murine IL-2 (0.67 mg/ml) and 7.5 μg anti-IL-2 mAb JES6-1A12 (3 mg ml, BioXCell) per mouse. The IL-2/α-IL-2 mixture was incubated at 37 °C for 30 min, adjusted to 200 μl/mouse with sterile PBS and injected into mice. Mice were sacrificed 1 day post injection.
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