Wild-type K-12 strain NCM3722 of E. coli45 (link) was cultured in Gutnick minimal complete medium46 (link) with 4g/L of glucose, glycerol, or acetate as the carbon source. Growth of cells was as previously reported26 (link). Preparation of filter cultures is described in Supplementary Methods online. For 13C-glucose cultures, uniformly 13C-glucose (> 99% 13C from Cambridge Isotope Laboratories) was used for the overnight culture and the liquid and filter cultures, resulting in at least 10 doublings on uniformly 13C-glucose media.
Protocol detail
Cultivation of K-12 E. coli with Carbon Sources
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Princeton University, Genomatica (United States)
Top 5 similar protocols
Protocol cited in 7 other protocols
Variable analysis
independent variables
- Carbon source (glucose, glycerol, or acetate)
- Growth of cells
- Gutnick minimal complete medium
- Concentration of carbon source (4g/L)
- Positive control: Wild-type K-12 strain NCM3722 of E. coli
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!