Myocardial Infarction Mouse Models

All animal procedures were approved by the Imperial College Governance Board for Animal Research and the Home Office (UK) and The Jackson Laboratory Institutional Animal Care and Use Committee, respectively. Mouse strains used in this study were B6(Cg)-Clec9a^tm1.1Crs/J (CLEC9a^−/−), B6.129S2-Cd8a^tm1Mak/J (CD8^−/−), and C57BL/6J (wild type [WT]). T1MI was induced by permanent ligation of the left anterior descending artery as previously described^{30 (link)} in male and female mice 10 to 12 weeks of age. T2MI was induced by a single intraperitoneal injection at a dose of 160 mg/kg isoproterenol HCL (Sigma-Aldrich, St. Louis, MO), as described previously.³¹ Monitoring of cardiac function was performed by transthoracic echocardiography by a blinded operator using a high-frequency ultrasound system Vevo 770 (VisualSonics Inc., Toronto, Canada) with a 30-MHz linear transducer.

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Forte E., Perkins B., Sintou A., Kalkat H.S., Papanikolaou A., Jenkins C., Alsubaie M., Chowdhury R.A., Duffy T.M., Skelly D.A., Branca J., Bellahcene M., Schneider M.D., Harding S.E., Furtado M.B., Ng F.S., Hasham M.G., Rosenthal N, & Sattler S. (2020). Cross-Priming Dendritic Cells Exacerbate Immunopathology After Ischemic Tissue Damage in the Heart. Circulation, 143(8), 821-836.

Publication 2020

isoproterenol HCL

Animal Artery Cardiac Female Institutional animal care and use committee Intraperitoneal injection Isoproterenol hcl Ligation Male Mice Strains Transducer Transthoracic echocardiography Ultrasound

Corresponding Organization :

Other organizations : Jackson Laboratory, Lung Institute, Imperial College London, Amgen (United States)

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Variable analysis

independent variables

Mouse strains: B6(Cg)-Clec9atm1.1Crs/J (CLEC9a−/−), B6.129S2-Cd8atm1Mak/J (CD8−/−), and C57BL/6J (wild type [WT])
T1MI induction by permanent ligation of the left anterior descending artery
T2MI induction by a single intraperitoneal injection of 160 mg/kg isoproterenol HCL

dependent variables

Cardiac function monitored by transthoracic echocardiography

control variables

Male and female mice 10 to 12 weeks of age

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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