The fractions were immunoblotted as described previously (Komatsu et al., 2001 (link)). The antibodies for Atg7 (Tanida et al., 1999 (link)) and Atg5 (Mizushima et al., 2001 (link)) were described previously. The antibodies for ubiquitin (DakoCytomation) and actin (MAB1501R; Chemicon International, Inc.) were purchased. The antibodies against LC3, GABARAP, and GATE-16 were raised in rabbits using their specific peptides as antigens. The antibodies against p112, Mss1, and α5 were provided by K.B. Hendil (August Krogh Institute, University of Copenhagen, Copenhagen, Denmark).
Protocol detail
Immunoblotting of Autophagy Proteins
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Tokyo Metropolitan Institute of Medical Science, Juntendo University, Osaka University, National Institute for Basic Biology
Top 5 similar protocols
Protocol cited in 30 other protocols
Variable analysis
independent variables
- Antibodies for Atg7, Atg5, ubiquitin, actin, LC3, GABARAP, GATE-16, p112, Mss1, and α5
- Protein expression levels of Atg7, Atg5, ubiquitin, actin, LC3, GABARAP, GATE-16, p112, Mss1, and α5
- Immunoblotting procedure described previously (Komatsu et al., 2001)
- None specified
- None specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!