MeJA-Induced Transcriptional Changes

Total RNA isolated from the SZ of stage S6 cm to S9 cm treated with 0 or 50 μM MeJA (Bioduly, Nanjing, China) for 48 h. The cDNA was prepared using a HiScript II kit 117 (Vazyme, Nanjing, China). Quantitative RT-qPCR reactions were conducted using ChamQ Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China) following the manufacturer’s protocol. Primers for the genes were designed using Primer express 3.0 software (Table S6). Gene expression levels were normalized using the reference gene 18S [5 (link)], and values were calculated using the 2^−ΔΔCt method [70 (link),71 (link)]. Expression values were obtained from three biological experiments. SPSS 17.0 statistical software was used to determine statistical significance.

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He L., Liu X., Wu Z, & Teng N. (2021). Transcriptome and Metabolome Analyses Provide Insights into the Stomium Degeneration Mechanism in Lily. International Journal of Molecular Sciences, 22(22), 12124.

Publication 2021

HiScript II kit 117 ChamQ Universal SYBR qPCR Master Mix SPSS 17.0 statistical

Biological Cdna Gene Gene expression Primer

Corresponding Organization : Ministry of Agriculture and Rural Affairs

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Variable analysis

independent variables

Treatment with 0 or 50 μM MeJA

dependent variables

Gene expression levels

control variables

Total RNA isolated from the SZ of stage S6 cm to S9 cm
CDNA prepared using a HiScript II kit
Quantitative RT-qPCR reactions conducted using ChamQ Universal SYBR qPCR Master Mix
Gene expression levels normalized using the reference gene 18S

controls

Positive control: Not explicitly mentioned
Negative control: Treatment with 0 μM MeJA

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