Genetic Diversity Analysis of A/H9N2 Influenza Viruses

Publicly available sequences of the A/H9N2 viruses from Myanmar and reference A/H9N2 strains were downloaded from the Global Initiative on Sharing All Influenza Data (GISAID; accession numbers are available in Table S1).¹¹ BLASTN homology analysis of each segment was performed on the GISAID website, and globally related sequences were downloaded. The evolutionary history of the sequences was inferred by using the Maximum Likelihood method and Kimura 2‐parameter model,¹² which were conducted in MEGA X.¹³ Pairwise sequence analyses were performed with Jalview¹⁴; reference sequences included A/H9N2 sequences collected in Myanmar in 2015, HI anti‐serum strains and other related sequences from the region.